Do data resources managed by EMBL-EBI and our collaborators make a difference to your work? Please take 10 minutes to fill in our annual user survey, and help us make the case for why sustaining open data resources is critical for life sciences research.
Structure of the formin Cdc12 bound to the barbed end of phalloidin-stabilized F-actin.
Resolution: 3.56 Å
EM Method: Single-particle
Fitted PDBs: 8rtt
Q-score: 0.444
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
To Be Published
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of phalloidin stabilized f-actin. (Complex)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Magnesium ion (24 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Phalloidin. (Complex from Amanita phalloides)
- Cell division control protein 12 (48 kDa, Protein from Schizosaccharomyces pombe)
- Actin filament (Complex from Homo sapiens)
Cryo-EM reconstruction of the formin Cdc12 bound to the barbed end of F-actin (without phalloidin)
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Human cytoplasmic beta-actin (Protein from Homo sapiens)
- Complex of the dimeric fh2 domain of s. pombe cdc12 bound to the barbed end of f-actin. (Complex from Homo sapiens)
- Dimeric fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- S. pombe cdc12 (Protein from Schizosaccharomyces pombe)
- Actin filament (Complex)
Resolution: 6.25 Å
EM Method: Single-particle
Fitted PDBs: 8rty
Q-score: 0.169
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Profilin-1-s29c/s71m (Complex from Homo sapiens)
- Actin-formin-profilin ring complex: the phalloidin-stabilized f-actin barbed end bound by dimeric-cdc12 and profilin-s71m. (Complex)
- Methylated-dna--protein-cysteine methyltransferase,cell division control protein 12 (77 kDa, Protein from Schizosaccharomyces pombe)
- Profilin-1 (15 kDa, Protein from Homo sapiens)
- Phalloidin (amanita phalloides) (808 Da, Protein from Amanita phalloides)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Phalloidin (Complex from Amanita phalloides)
- Dimeric fh1fh2 domain of s. pombe cdc12 (Complex from Schizosaccharomyces pombe)
- Actin filament (Complex from Homo sapiens)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
Structure of the undecorated barbed end of F-actin.
Resolution: 3.08 Å
EM Method: Single-particle
Fitted PDBs: 8ru0
Q-score: 0.542
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Complex of the actin subunits that form the barbed end of actin filaments. (Complex from Oryctolagus cuniculus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Phosphate ion (94 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Actin filament (Complex)
Structure of the F-actin barbed end bound by formin mDia1
Resolution: 3.49 Å
EM Method: Single-particle
Fitted PDBs: 8ru2
Q-score: 0.362
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Mdia1-bound f-actin barbed end. (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Methylated-dna--protein-cysteine methyltransferase,protein diaphanous homolog 1 (86 kDa, Protein from Mus musculus)
- Magnesium ion (24 Da, Ligand)
- Mouse mdia1 (fh1fh2c domain) (Complex from Mus musculus)
- Actin filament (Complex from Homo sapiens)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
Structure of the formin INF2 bound to the barbed end of F-actin.
Resolution: 3.41 Å
EM Method: Single-particle
Fitted PDBs: 8rv2
Q-score: 0.43
Oosterheert W, Boiero Sanders M, Funk J, Prumbaum D, Raunser S, Bieling P
Science (2024) 384 pp. eadn9560-eadn9560 [ DOI: doi:10.1126/science.adn9560 Pubmed: 38603491 ]
- Actin filament barbed end (Complex from Oryctolagus cuniculus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Complex of the formin inf2 dimer that binds to the actin subunits at the barbed end of actin filaments. (Complex)
- Isoform 2 of inverted formin-2 (84 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Phosphate ion (94 Da, Ligand)
- Inf2 dimer (Complex from Homo sapiens)
Cryo-EM structure of ADP-bound, filamentous beta-actin harboring the R183W mutation
Resolution: 2.28 Å
EM Method: Single-particle
Fitted PDBs: 8oi8
Q-score: 0.679
Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Nat Struct Mol Biol (2023) 30 pp. 1774-1785 [ Pubmed: 37749275 DOI: doi:10.1038/s41594-023-01101-9 ]
- Water (18 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from Homo sapiens)
- Actin filament harboring the r183w mutation. (Complex from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of the undecorated barbed end of filamentous beta/gamma actin
Resolution: 3.59 Å
EM Method: Single-particle
Fitted PDBs: 8oi6
Q-score: 0.464
Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Nat Struct Mol Biol (2023) 30 pp. 1774-1785 [ Pubmed: 37749275 DOI: doi:10.1038/s41594-023-01101-9 ]
- Phalloidin (Complex from Amanita phalloides)
- Actin-phalloidin complex (Complex)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Cytosolic beta-gamma actin (Complex from Bos taurus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Actin, cytoplasmic 1 (41 kDa, Protein from Bos taurus)
Cryo-EM structure of ADP-bound, filamentous beta-actin harboring the N111S mutation
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8oid
Q-score: 0.681
Oosterheert W, Blanc FEC, Roy A, Belyy A, Sanders MB, Hofnagel O, Hummer G, Bieling P, Raunser S
Nat Struct Mol Biol (2023) 30 pp. 1774-1785 [ Pubmed: 37749275 DOI: doi:10.1038/s41594-023-01101-9 ]
- Water (18 Da, Ligand)
- Actin filament harboring the n111s mutation. (Complex from Homo sapiens)
- Actin, cytoplasmic 1, n-terminally processed (41 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
Structure of P. luminescens TccC3-F-actin complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7z7h
Q-score: 0.366
Belyy A, Lindemann F, Roderer D, Funk J, Bardiaux B, Protze J, Bieling P, Oschkinat H, Raunser S
Nat Commun (2022) 13 pp. 4202-4202 [ DOI: doi:10.1038/s41467-022-31836-w Pubmed: 35858890 ]
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- Nicotinamide (122 Da, Ligand)
- Adenosine-5-diphosphoribose (559 Da, Ligand)
- Structure of p. luminescence tccc3-f-actin complex (Complex from Photorhabdus luminescens)
- Maltose/maltodextrin-binding periplasmic protein,tccc3 (63 kDa, Protein from Photorhabdus luminescens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)